diff --git a/moto/ec2/models.py b/moto/ec2/models.py
index a450943b..cc333e79 100755
--- a/moto/ec2/models.py
+++ b/moto/ec2/models.py
@@ -388,6 +388,7 @@ class Instance(TaggedEC2Resource, BotoInstance):
         self.ebs_optimized = kwargs.get("ebs_optimized", False)
         self.source_dest_check = "true"
         self.launch_time = utc_date_and_time()
+        self.ami_launch_index = kwargs.get("ami_launch_index", 0)
         self.disable_api_termination = kwargs.get("disable_api_termination", False)
         self._spot_fleet_id = kwargs.get("spot_fleet_id", None)
         associate_public_ip = kwargs.get("associate_public_ip", False)