diff --git a/moto/core/utils.py b/moto/core/utils.py
index 65f0f457..c72cb643 100644
--- a/moto/core/utils.py
+++ b/moto/core/utils.py
@@ -74,6 +74,10 @@ def convert_regex_to_flask_path(url_path):
         return '<regex("{0}"):{1}>'.format(match_pattern, match_name)
 
     url_path = re.sub("\(\?P<(.*?)>(.*?)\)", caller, url_path)
+
+    if url_path.endswith("/?"):
+        # Flask does own handling of trailing slashes
+        url_path = url_path.rstrip("/?")
     return url_path
 
 
diff --git a/tests/test_s3bucket_path/test_bucket_path_server.py b/tests/test_s3bucket_path/test_bucket_path_server.py
index d639c4ce..4434c02f 100644
--- a/tests/test_s3bucket_path/test_bucket_path_server.py
+++ b/tests/test_s3bucket_path/test_bucket_path_server.py
@@ -21,23 +21,23 @@ def test_s3_server_bucket_create():
     backend = server.create_backend_app("s3bucket_path")
     test_client = backend.test_client()
 
-    res = test_client.put('/foobar/', 'http://localhost:5000')
+    res = test_client.put('/foobar', 'http://localhost:5000')
     res.status_code.should.equal(200)
 
     res = test_client.get('/')
     res.data.should.contain(b'<Name>foobar</Name>')
 
-    res = test_client.get('/foobar/', 'http://localhost:5000')
+    res = test_client.get('/foobar', 'http://localhost:5000')
     res.status_code.should.equal(200)
     res.data.should.contain(b"ListBucketResult")
 
-    res = test_client.get('/missing-bucket/', 'http://localhost:5000')
+    res = test_client.get('/missing-bucket', 'http://localhost:5000')
     res.status_code.should.equal(404)
 
-    res = test_client.put('/foobar/bar/', 'http://localhost:5000', data='test value')
+    res = test_client.put('/foobar/bar', 'http://localhost:5000', data='test value')
     res.status_code.should.equal(200)
 
-    res = test_client.get('/foobar/bar/', 'http://localhost:5000')
+    res = test_client.get('/foobar/bar', 'http://localhost:5000')
     res.status_code.should.equal(200)
     res.data.should.equal(b"test value")
 
@@ -46,14 +46,14 @@ def test_s3_server_post_to_bucket():
     backend = server.create_backend_app("s3bucket_path")
     test_client = backend.test_client()
 
-    res = test_client.put('/foobar2/', 'http://localhost:5000/')
+    res = test_client.put('/foobar2', 'http://localhost:5000/')
     res.status_code.should.equal(200)
 
-    test_client.post('/foobar2/', "https://localhost:5000/", data={
+    test_client.post('/foobar2', "https://localhost:5000/", data={
         'key': 'the-key',
         'file': 'nothing'
     })
 
-    res = test_client.get('/foobar2/the-key/', 'http://localhost:5000/')
+    res = test_client.get('/foobar2/the-key', 'http://localhost:5000/')
     res.status_code.should.equal(200)
     res.data.should.equal(b"nothing")